Extended Data Fig. 2: hDPP4 inhibitors act on BT4193 but not BT3254.
From: Chemoproteomic identification of a DPP4 homolog in Bacteroides thetaiotaomicron
a, Quantification of percent inhibition of GP-AMC cleavage in wild type (WT) B. thetaiotaomicron lysate (ex/em: 380/460 nm) after pretreatment with inhibitor for 30 min at 37 °C. Activity was normalized to DMSO treatment (mean ± SEM; n = 6 independent replicates). b, Apparent IC50 values of recombinant hDPP4 and BT4193 after treatment with inhibitor for 30 min prior to measuring activity via GP-AMC cleavage and the corresponding curves. Activity was normalized to DMSO treatment (100%) and no enzyme controls (0%), and fit with dose-dependent four parameter inhibition. Selectivity index (SI) is the ratio of BT4193 to hDPP4 apparent IC50 values (mean ± SD; n = 2 independent experiments, each calculated with 3 independent replicates). c, Quantification of inhibition of recombinant BT3254 by hDPP4 inhibitors after 30 min of pretreatment. Velocities were normalized to DMSO pretreatment. Data represent the mean ± SEM of 9 independent replicates.
