Extended Data Fig. 6: Membrane permeable β1AR selective agonist, dobutamine, promotes PLB phosphorylation in adult OCT3 knock-out cardiomyocytes.
From: Cardiac contraction and relaxation are regulated by distinct subcellular cAMP pools
a. Representative western blots of adult cardiomyocytes derived from wild-type or OCT3 knock-out stimulated with 0.3 or 3 μM dobutamine (Dob). b-d. Quantification of Western blot of p-RyR2 Ser2808, p-TnI Ser23/24, and p-PLB Ser16/Thr17 normalized to the protein level of CSQ2, and then reported as a percentage of the highest value in the groups. The quantified data from different experiments were presented as mean ± S.E.M. The p-values were calculated by two-tailed t test. n = 6 biological replicates.
