Extended Data Fig. 7: Epinephrine-mediated Ca2+ decay tau was delayed in adult cardiomyocytes derived from OCT3 knock-out mice.
From: Cardiac contraction and relaxation are regulated by distinct subcellular cAMP pools
a. Adult cardiomyocytes were loaded with 5 μM Ca2+ indicator (Fluo-4 AM). Representative line scan images and the corresponding trace of fluorescence obtain from epinephrine-mediated Ca2+ response upon 1 Hz field stimulation in adult cardiomyocytes derived from wild-type (left) and OCT3 knock-out mice (right). b, c. Ca2+ transient amplitude, and d. decay tau were recorded with 1 Hz pacing and upon 1 μM epinephrine stimulation for 5 minutes in adult cardiomyocytes derived from wild-type and OCT3 knock-out mice. The quantified data from different experiments were presented as mean ± S.E.M. The p-values were calculated by two-way ANOVA. n = 6 and 7 cells for amplitude and decay tau, respectively, 3 biological replicates.
