Fig. 3: Plasma membrane and the Golgi pools of β1AR function differently in adult mouse cardiomyocytes.
From: Cardiac contraction and relaxation are regulated by distinct subcellular cAMP pools
a, Representative western blots of phosphorylation profiles of RyR2, TnI and PLB regulated by β1AR in adult cardiomyocytes derived from WT mice, treated with 10 μM corticosterone (Cortico). Adult cardiomyocytes were pretreated with 10 μM β2AR-selective antagonist ICI-118551 (ICI) to isolate the function of β1ARs. Membrane-permeable β1AR-selective agonist, dobutamine (Dob), promotes PLB phosphorylation independent of OCT3. b–d, Quantification of immunoblots of pRyR2 Ser2808, p-TnI Ser23/Ser24 and pPLB Ser16/Thr17 normalized to the protein level of CSQ2 and then reported as a percentage of the highest value in the groups. The quantified data from different experiments were presented as mean ± s.e.m. The P values were calculated by one-way ANOVA. n = 6 biological replicates. e, Representative western blots of phosphorylation profiles of RyR2, TnI and PLB regulated by β1AR in adult cardiomyocytes derived from OCT3 (SLC22A3) knockout mice and compared to WT. f–h, Quantification of immunoblots of pRyR2 Ser2808, pTnI Ser23/Ser24 and pPLB Ser16/Thr17 normalized to the protein level of CSQ2 and then reported as a percentage of the highest value in the groups. The quantified data from different experiments were presented as mean ± s.e.m. The P values were calculated by two-way ANOVA. n = 5 and 7 biological replicates for WT and OCT3 knockout cardiomyocytes, respectively.
