Fig. 6: Pressure–volume measurement of OCT3 knockout mice revealed preserved systole but impaired diastole upon epinephrine stimulation.
From: Cardiac contraction and relaxation are regulated by distinct subcellular cAMP pools
a, Diagram demonstrating the placement of the catheter for pressure–volume measurements in mouse hearts (top). Representative pressure–volume loop shows the changes in pressure and volume during isovolumetric contraction. Multiple cardiac indicators, including the end-systolic pressure–volume relationship (ESPVR), end-diastolic pressure–volume relations (EDPVR), stroke volume, cardiac output and ejection fraction, can be derived from PV loops (bottom). b–e, Representative hemodynamic pressure–volume loops (five loops) upon 10 μg kg−1 epinephrine injection in (b) WT and (c) OCT3 (SLC22A3) knockout mice or 18.4 μg kg−1 dobutamine injection in (d) WT and (e) OCT3 knockout mice. f, The maximum dP/dt derived from the PV loops (a measurement of systolic function). g, The minimum dP/dt (a measurement of diastolic function), upon epinephrine (10 μg kg−1) or dobutamine (18.4 μg kg−1) bolus injection through the jugular vein of WT and OCT3 (SLC22A3) knockout mice. h, Tau represents the decay of pressure during isovolumetric relaxation that is preload independent. Data are presented as mean ± s.d. The P values were calculated by two-tailed t-test. n = 10 WT and OCT3 knockout mice for epinephrine treatment and n = 7 WT and 10 OCT3 knockout mice for dobutamine treatment.
