Extended Data Fig. 6: Sample traces and concentration-response curves for WT and FMRFamide coordinating residues mutants of AcFaNaC.
From: Structure and mechanism of a neuropeptide-activated channel in the ENaC/DEG superfamily
Sample traces and concentration-response curve for each construct is boxed. For sample traces, increasing concentration of FMRFamide was applied by perfusion as indicated by bars. Concentration-response curves of mutant were measured using multiple independent cells (n = 7 (I147A), n = 6 (L168A), n = 5 (Q172A), n = 6 (N183A), n = 5 (F188A), n = 5 (T271A), n = 9 (Y272A), n = 8 (V274A), n = 7 (F453A)), and the curve for each mutant is shown as a solid line and is overlaid with that of WT, which is shown as dashed line. All data points are mean ± SEM.
