Extended Data Fig. 7: Metal dependence of ZmpB for activity against 1OGO-9.
From: A high-throughput screening platform for enzymes active on mucin-type O-glycoproteins
a, ZmpB was assayed for activity against 1minOGO-1 across a range of concentrations of ZnCl2 in 100 mM MES pH 6.5. Data points indicate mean of three (for 1μΜ and 25 nM data points) or six technical replicates (for all other data points). Error bars indicate standard error of the mean. b, Metal-dependence of ZmpB was further investigated by assaying activity of ZmpB where metals or EDTA were added to a final concentration of 25 μM. Values shown are technical replicates relative to the activity of ZmpB in the presence of 25 μM ZnCl2.
