Extended Data Fig. 8: Model sort to show efficacy of FACS-based enrichment for clones with active O-glycopeptidase and O-glycosylation machinery.
From: A high-throughput screening platform for enzymes active on mucin-type O-glycoproteins
Cells expressing the probe, BT4244 and minOGO-1 were diluted 1:100 fold in cells expressing the probe, BT4244, and the equivalent empty vector to minOGO-1 (such that no glycosylation occurs). a, These were then sorted by FACS. Shown is the data for the separate populations (that is, without dilution of one set of cells into the other) as well as the gate used for sorting. Label indicates % of cells within the sorting gate. b, Ten recovered clones were then tested for functional glycosylation and O-glycopeptidase activity by individual culturing, expression, lysis, and then analysis by SDS-PAGE followed by detection using in gel fluorescence.
