Extended Data Fig. 3: Metal dependence of IMPa for activity against 1minOGO-1.
From: A high-throughput screening platform for enzymes active on mucin-type O-glycoproteins
a, IMPa was assayed for activity against 1minOGO-1 across a range of concentrations of ZnCl2 in 100 mM MES pH 6.5. kcat/KM was determined via the substrate depletion method as previously described6 and/or using the initial reaction velocity as indicated. Data points indicate mean of three technical replicates. Error bars represent the standard error of the mean. b, Metal-dependence of IMPa was further investigated by assaying activity of IMPa where metals or EDTA were added to a final concentration of 100 μM. Values shown are technical replicates relative to the activity in the presence of 100 μM ZnCl2.
