Extended Data Fig. 4: Cleavage of different glycoforms of probe 1 by BT4244.
From: A high-throughput screening platform for enzymes active on mucin-type O-glycoproteins
Probe 1 was expressed with different OGOs in order to produce a variety of different glycoforms. BT4244 was then assayed for activity against these different glycoforms. This was observed through (a) the change in FRET over time and (b) SDS-PAGE followed by Coomassie staining. Data points shown in panel a correspond to four technical replicates for +BT4244 samples and three technical replicates for Buffer Control samples. For panel b, the label ‘m.’ indicates probe co-expressed with minOGO-1.
