Extended Data Fig. 1: OGT binding evaluation of motif-containing peptides.
From: Motif-dependent binding on the intervening domain regulates O-GlcNAc transferase
(a) Sequences of one consensus (CP37) and seven natural motif-containing peptides along with their apparent binding affinities (EC50) from competitive fluorescence polarization assay using 5-FAM-SMG9 peptide and OGT4.5. Standard deviations from three biological replicates are shown. PxYx[I/L] motif in each peptide is highlighted in red. (b) Thermal shift assay showing the shift in denaturation temperature (Tm) of OGT and OGT4.5 with peptide incubation, n = 3. (c) Competitive fluorescence polarization assay between 5-FAM-SMG9 and motif-containing peptides AK9 (blue), DENND1B (green), OR2AG1 (purple), JMJD1C (brown), and CAMKMT (cyan) for OGT4.5 binding. Data are presented as individual measurements ± SD of mean of three biological replicates.
