Extended Data Fig. 9: Differential HDX-MS analysis of NMM-bound NicA2 v321 vs wildtype.
From: Directed evolution unlocks oxygen reactivity for a nicotine-degrading flavoenzyme
a, Peptide coverage map, showing the common peptides detected for wild-type and NicA2 v321. Peptides where the mutations are present are not included in these analyses. b, Woods’ plots showing the difference in deuterium uptake between peptides derived from wild-type or NicA2 v321 when bound to NMM for the 0.5, 2, 5 min timepoints. Peptides that showed statistically significant deprotection in v321 are colored in red. No regions of protection from exchange were detected. The vertical thin blue lines represent the location of mutations between NicA2 wildtype and v321.
