Extended Data Fig. 1: kcat/KM is not reliably selected for under plate selection conditions.
From: Directed evolution unlocks oxygen reactivity for a nicotine-degrading flavoenzyme
a, The top panel is a recreation of Fig. 1c with additional labels for comparison. Frequently mutated residues interrogated in Fig. 2 are bolded. b, kcat/KM values for variants listed in Supplementary Table 1 plotted based on the generation of the selection they were isolated from. The color scheme holds between this figure and Fig. 1 of the main text.
