Extended Data Fig. 2: Alignment of NicA2 variants tested for steady-state kinetic activity.
From: Directed evolution unlocks oxygen reactivity for a nicotine-degrading flavoenzyme
Red asterisks denote amino acid locations found to be mutated in greater than 15% of all sequences isolated from the selection. Sequences are colored for their degree of conservation using the BLOSUM62 score. Figure was prepared using Jalview57.
