Table 1 Kinetic properties of NicA2 enzymes
From: Directed evolution unlocks oxygen reactivity for a nicotine-degrading flavoenzyme
Enzyme | kcat | Ligand-free \({k}_{{{\mathrm{ox}}}}^{{{\mathrm{O}}}_{2}}\) | NMM-bound \({k}_{{{\mathrm{ox}}}}^{{{\mathrm{O}}}_{2}}\) | kred,1 | kred,2 |
|---|---|---|---|---|---|
Wild type | 0.0066 s−1 | 120 M−1 s−1 | 28 M−1 s−1 | 800 s−1 | 120 s−1 |
v320 | 1.1 s−1 | 230 M−1 s−1 | 4,400 M−1 s−1 | 19 s−1 | 4.1 s−1 |
v321 | 0.58 s−1 | 230 M−1 s−1 | 2,000 M−1 s−1 | 130 s−1 | 10 s−1 |
