Extended Data Fig. 7: Biosynthesis of halo-strictosidine by MIA-CM3 and MIA-CM10.
From: Biosynthesis of natural and halogenated plant monoterpene indole alkaloids in yeast
MIA-CM3 and MIA-CM10 were cultivated (see Methods) with 100 mg/L haloindole and 0.25 mM secologanin and the broth at 144 hours analyzed using HRMS. a. Representative chromatograms for MIA standards and fluorinated, chlorinated, brominated and difluorinated derivatives after feeding the corresponding haloindole. b. Representative MS-MS spectra extracted from the corresponding peaks in panel a. c,d, Indole to strictosidine pathways expressed in MIA-CM3 (c) and MIA-CM10 (d) with the detection of halogenated derivatives overlaid. The presence of a colored or gray box indicates identification of the derivative with a halo-substitution(s) at the indicated carbon (indole numbering). Enzyme abbreviation: TRP5 - tryptophan synthase 5, TDC - tryptophan synthase, STR - strictosidine synthase.
