Fig. 2: Bioactivity testing of alstonine in yeast and mammalian cells.
From: Biosynthesis of natural and halogenated plant monoterpene indole alkaloids in yeast
a, Dose–response curves of ADRA2A with the agonist epinephrine (left; R2 = 0.988) and antagonist activities of yohimbine and alstonine (right; R2 = 0.965 and 0.980, respectively). Data are reported in relative luminescence units (RLU) based on nano-luciferase (NanoLuc) readouts normalized to the maximum observed luminescence. Data report triplicate biological replicates (n = 3) measured on yeast cells heterologously expressing the ADRA2A receptor; [epinephrine], concentration of epinephrine; [antagonist], concentration of antagonist; [serotonin], concentration of serotonin; [alstonine], concentration of alstonine. b, Dose–response curves of 5-HT2C with the agonist serotonin (left; R2 = 0.892) and alstonine with and without 0.5 µM competing serotonin (right; R2 = 0.792 and 0.913). cAMP levels were monitored using BRET in COS7 cells, and data are reported for biological duplicates (n = 2). For all data presented, each replicate is shown, and a non-linear regression model was applied using the least squares method. The dashed lines in a and b (left) indicate 50 µM and 0.5 µM, respectively, which were the agonist concentrations used in the respective competitor assays (right).
