Extended Data Fig. 6: Recorded SNP sequences extracted from consortium samples by NGS.

Consortium of all five sentinel cells (B. subtilis SEN1/SEN2/SEN3/SEN4/SEN5) were incubated with mixtures of all five target DNA’s (+SEQ1/SEQ2/SEQ3/SEQ4/SEQ5 in replicate 1; +SEQ1^G/SEQ2^C/SEQ3^G/SEQ4^T/SEQ5^A in replicate 2; +SEQ1/ SEQ2^C/SEQ3^G/SEQ4^G/SEQ5 in replicate 3), as indicated above each row. Sequence logos were generated from the reads aligned at the SNP position of recorder constructs with the terminator excised (recorder sequences SEN1^ΔDT, 2^ΔDT, 3^ΔDT, 4^ΔDT, 5^ΔDT). The SNP position is highlighted with bold text. In all cases, the SNP extracted from NGS reads matched the SNP present on the target DNA’s added (the SNPs on SEQ1, SEQ2, SEQ3, SEQ4, and SEQ5 are T, T, A, A, G, respectively). The data show three replicates performed on different days.