Extended Data Fig. 3: Response to homologous animal sequences.
From: Sentinel cells programmed to respond to environmental DNA including human sequences
Species-level phylogenetic trees for animals with sequences homologous to SEQ2, SEQ3, and SEQ4. No homologous sequences were identified for SEQ5. Schematics comparing homologous animal sequences to the original show sequence identities (orange – SEQ2 homologs; purple – SEQ3 homologs; green – SEQ4 homologs); mismatches (red), insertions (red ticks above bar), and deletions (gaps in bar). The percent identity of the animal homolog sequence to the original, as determined by the Needleman-Wunsch algorithm, is shown above each sequence. The data show the fraction ON for each sentinel cell incubated with 800 pM of each target DNA, calculated from the data in Supplementary Fig. 1. The data points for 100% sequence identity used human SEQ2/SEQ2C, SEQ3/SEQ3G, and SEQ4/SEQ4G/SEQ4T for B. subtilis SEN2, SEN3, and SEN4, respectively, and were identical to the data shown in Extended Data Fig. 2 at 800pM. The dashed line is identical to the dashed line shown in the DNA recording frequency plots in Extended Data Fig. 2 and shows the mean DNA recording frequency calculated from sentinel cells incubated without DNA. The data show three replicates performed on different days and the solid lines are the mean values.
