Extended Data Fig. 6: Testing the activity of SlugCas9-N984S/K1016I using the GFP-activation reporter in HEK293T cells.
From: Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM
a, Schematic of the GFP-activation reporter. Dark lines indicate target sites; PAMs are indicated by red lines. b, Percentage of GFP-positive cells after genome editing by SlugCas9 or SlugCas9-N984S/K1016I. For the gating strategy, begin by drawing the FSC/SSC dual-parameter dot map, then encircle the target cell population and establish the gate R1. Subsequently, draft the GFP/SSC dual-parameter dot map. After applying Gate R1 to this map, set a gate to encircle GFP positive cells, R2, using the negative control as a reference. Two-tailed, paired Student’s t-test was used to determine the statistical significance between the two groups. A value of P < 0.05 was considered to be statistically significant (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Data are mean + s.d. of n = 3 independent experiments. Statistical source data are available (Source Data Extended Data Fig. 6).
