Extended Data Fig. 1: SlugCas9 exhibits comparable editing activity to SpCas9.
From: Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM
a, Schematic of the Cas9 expression constructs. Both SpCas9 and SlugCas9 constructs have the same backbone. b, Expression levels of SpCas9 and SlugCas9 measured by RT-qPCR. Two-tailed, paired Student’s t-test was used to determine the statistical significance between the two groups. Data are mean + s.d. of n = 3 independent experiments. c, Schematic of sgRNA design. d, Indel frequency of SpCas9 and SlugCas9 at 23 endogenous target sites in HEK293T cells. Cas9 and sgRNA expression plasmids were transfected into HEK293T cells, followed by puromycin selection. Data are mean + s.d. of n = 3 independent experiments. e, Summary of the indel frequency of SpCas9 and SlugCas9. Two-tailed, paired Student’s t-test was used to determine the statistical significance between the two groups. The mean and s.d. of all individual values of n = 3 independent biological replicates are plotted. Statistical source data are available (Source Data Extended Data Fig. 1).
