Extended Data Fig. 3: Phage propagation level detected by PCR amplification.
From: Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM
a, PCR amplification of the phage genome (1,615 bp). 1 µL of filtered supernatant containing phages from the phage propagation assay is used as a template. Forward and reverse primers are indicated by red arrows. b, PCR products are analyzed by agarose gel electrophoresis. The expected PCR band size (1,615 bp) is indicated on the right by the black arrow. Ctr, a titer of 106 phages, is input into 2 ml of medium containing bacteria (OD600 = 0.5) and immediately filtered without culture. The PAM for each site is shown. The experiment was independently repeated three times and yielded similar results (Source Data Extended Data Fig. 3).
