Extended Data Fig. 3: LATS2 condensates mediate signaling and protein stability.
From: LATS2 condensates organize signalosomes for Hippo pathway signal transduction
a, Typical Confocal images of AD293 cells expressing indicated mEGFP fused Hippo pathway components (green) stained for nuclei (blue). b, Typical confocal images of AD293 cells co-expressing mEGFP-LATS2 (green) with TAZ forms (red) stained for nuclei (blue). c, Typical confocal images of MCF10A-LATS2-3XHA expressing indicated mEGFP fused Hippo pathway components treated by trypsin and stained for HA (red) and nuclei (blue). d, The histogram shows the quantitation of the ratio of cytoplasmic via nuclear YAP in Fig. 3c. n = 3, images acquired from three random views. e, Western blot assay for examining p-LATS and p-YAP in HEK293Tcells with indicated transfections of LATS2 forms. f, 3D simulation HA puncta by Imaris from Fig. 3e. Data are presented as mean ± SEM. Scale bars, 5 µm as indicated. β-actin was used for loading control.
