Extended Data Fig. 8: Immobilised GnTI-GalT cascade on monomeric Fc (mFc). | Nature Chemical Biology

Extended Data Fig. 8: Immobilised GnTI-GalT cascade on monomeric Fc (mFc).

From: Immobilized enzyme cascade for targeted glycosylation

Extended Data Fig. 8

MALDI-TOF MS profile of permethylated N-linked glycans derived from SuperMan5 P. pastoris-produced mFc. The first panel shows the glycomic spectrum of the mFc without enzymatic processing (control). Subsequent panels show the glycomic profile of the mFc derived N-glycans after sequential processing in SUGAR-TARGET with GnTI and GalT. All molecular ions represent the singly charged and sodiated form ([M + Na]+); VH: variable heavy chain; VL: variable light chain, CH1: constant heavy chain 1; CL: constant light chain; CH2: constant heavy chain 2; CH3: constant heavy chain 3; Fab: antigen binding fragment; Fc: crystallizable fragment.

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