Extended Data Fig. 5: Additional outcomes from two-week toxicology studies with hinokitiol and GT.
From: Minimizing higher-order aggregation maximizes iron mobilization by small molecules
a, Animal weights from two-week daily administration of hinokitiol at 10, 30 and 100 mg/kg/day (n = 6 animals per group). b, Pharmacokinetic study to establish bioavailability and approximate exposure to hinokitiol (n = 3 animals per group). c, Pharmacokinetics of hinokitiol administered on day 1 to rats (n = 3 animals per group). d, Pharmacokinetics of hinokitiol administered on day 15 to rats (n = 3 animals per group). e, Pharmacokinetic calculations from studies in c,d. f, Representative H&E staining of rat myocardium from an animal treated with 30 mg/kg of hinokitiol. g, Representative H&E staining of rat myocardium from an animal treated with 100 mg/kg of hinokitiol. h, Representative H&E staining of rat myocardium from an animal treated with 100 mg/kg of hinokitiol. i, Rat weights from two-week daily administration of GT at 15, 45 and 105 mg/kg/day (n = 6 animals per group). j, Pharmacokinetic study to establish bioavailability and approximate exposure to GT (n = 3 animals per group). k, Pharmacokinetic study of serum concentrations of GT on day 1 of the study (n = 3 animals per group). l, Pharmacokinetic study of serum concentrations of GT on day 15 of the study (n = 3 animals per group). m, Pharmacokinetic calculations from studies with GT in c,d. n, Representative H&E staining of rat myocardium from an animal treated with 45 mg/kg of GT. o, Representative H&E staining of rat myocardium from an animal treated with 105 mg/kg of GT. p, Representative DAB-Perl’s staining of rat myocardium from an animal treated with 105 mg/kg of GT. Arrows indicate features of interest. For panels f–h, n–p, the scale bars indicate 200 μm, and a minimum of three animals were processed and imaged for each treatment group. Pharmacokinetic calculations were performed in WinNonlin. Error bars indicate SEM.
