Extended Data Fig. 7: Additional data from three-month mouse toxicology studies with FeM-1269 and stress erythropoiesis follow-up.
From: Minimizing higher-order aggregation maximizes iron mobilization by small molecules
a, Male weights with daily administration of FeM-1269 (n = 6 animals per group). b, Female weights with daily administration of FeM-1269 (n = 6 animals per group). c,d, Histopathological analysis of tissues following FeM-1269 administration for 90 days in male and female mice (n = 6 animals per group). Only the tissues in which findings were observed were included in these graphs. Observation of the following tissues yielded no histopathological findings: brain, skeletal muscle, small intestine, spleen or thymus. Statistics in c,d were performed using two-way ANOVA with Dunnett’s multiple comparisons test. e, Female mouse weights following treatment with vehicle (0.5% HPMC) or 150 mg/kg/day of FeM-1269 (n = 12 animals per group). f, Spleen iron as assessed by ICP-MS (n = 12 per group, p = 0.0437). g, Histopathology analysis of mouse spleens using Prussian blue staining (n = 12 per group, p = 0.1114). h, Counts of bone marrow burst-forming unit, erythrocytes (BFU) (n = 12 per group, p = 0.4848). i, Counts of bone marrow colony-forming unit, erythrocytes (CFU) (n = 12 per group, p = 0.6243). j, Counts of spleen-derived BFU (n = 12 per group, p = 0.9999). k, Counts of bone marrow burst-forming unit, erythrocytes (CFU) (n = 12 per group, p = 0.0934). Statistics in f–k were performed using two-sided unpaired t-tests with Welch’s correction. For p-values for c,d, please see the source data file. Error bars represent SEM.
