Extended Data Fig. 1: In vitro iron mobilization and aggregation by tropolonoids.
From: Minimizing higher-order aggregation maximizes iron mobilization by small molecules
a, Time course of iron efflux from liposomes with different concentrations of hinokitiol, GT or FeM-1269 (n = 3; representative experiment shown). b, Rates of iron efflux from liposomes at each concentration of compounds, calculated from data in a–c. c, DLS derived count rate measured in different concentrations of MES/TRIS buffer, pH 7.0 (n = 3; representative experiment shown). d, DLS derived count rate measured at different pH of 50 mM MES/TRIS buffer (n = 3; representative experiment shown). e, DLS derived count rate measured with different buffering agents at 50 mM of buffer (n = 3; representative experiment shown). f, DLS derived count rate of hinokitiol2:Cu(II), GT2:Cu(II) and FeM-12692:Cu(II) (n = 3; representative experiment shown). g, Additional representative images from 90 μM of hino3:Fe(III), GT3:Fe(III) or FeM-12693:Fe(III) in buffer (n = 50, 45, 51 images for hino, GT and FeM-1269, respectively; images were taken from three independent sample preparations; scale bar is 500 nm). h, Iron efflux by tropolone derivatives from shDMT1 Caco-2 epithelia at 1 μM (n = 3, representative experiment shown). i, Iron efflux by tropolone derivatives from shDMT1 Caco-2 epithelia at 5 μM (n = 3, representative experiment shown).
