Extended Data Fig. 6: Prominent soma biotinylation by Cal-ID.
From: Molecular recording of calcium signals via calcium-dependent proximity labeling
(a) Subcellular fractionation of Cal-ID-biotinylated primary cortical neurons at DIV 21. T: cpTurboID, C: Cal-ID. Independent measurements: n = 3. Numbers on the left: molecular weight in kDa. *: endogenous biotin carrier proteins, #: self-biotinylation of Cal-ID or cpTurboID. PSD-95, GluA1/2: postsynaptic markers, Synaptophysin: presynaptic marker, V5: Cal-ID/cpTurboID epitope. biotin: 100 µM, 1 hour incubation. (b) Representative images of biotinylation patterns in neurons by Cal-ID or cpTurboID. Independent measurements: n = 6. Color map: signal intensity. Mouse primary hippocampal neurons at DIV 21. Scale bars: 100 µm. (c and d), Scatterplots showing mass spectrometry reproducibility between biological replicates (c) Cal-ID, (d) cpTurboID, prepared from mouse primary cortical neurons at DIV 21.
