Extended Data Fig. 7: Proximity ligation assay from mouse primary neurons.
From: Molecular recording of calcium signals via calcium-dependent proximity labeling
(a) A schematic diagram of proximity ligation assay (PLA) to visualize self-biotinylation of Cal-ID. (b) PLA was performed with V5 (Cal-ID) and biotin. Independent measurements: n = 3. Activated: 50 µM APV + 10 µM NBQX for overnight silencing, followed by inhibitor wash-off and addition of 4 mM CaCl2 along with 100 µM biotin for 1 hour. Mouse primary hippocampal neurons at DIV 21, lentiviral delivery, scale bar: 100 µm. (c) PLA experiments with PMCA1 or PMCA2 antibodies with biotin antibodies were conducted without Cal-ID expression (V5) in mouse primary hippocampal neurons at DIV 21. Independent measurements: n = 2. Scale bars: 50 µm.
