Extended Data Fig. 5: Effects of purines and GSH on IgG+ class switching in B cells.
From: Metabolic determinants of germinal center B cell formation and responses
a, Class switching of naive B cells activated by anti-CD40 + mIL4 for 72 h in the medium with different doses of purines and/or GSH as indicated. Cells per group from n = 3 mice. b, c, Relative to Extended Data Fig. 5a. Cell proliferation and survival were detected by CFSE staining (b) and PI staining (c) respectively. d, Surface expression of GL7 and Fas on iGBs generated from Mthfd2+/+ and Mthfd2−/− B cells (n = 3 independent wells per treatment). e,f, Naive B cells were activated with anti-CD40 + mIL4 in the medium containing 200 μM adenosine monophosphate-13C10, 15N5 or 200 μM Guanosine-5 ‘- monophosphate-15N5 sodium for 24 h. The levels of labelled indicated metabolites in B cells were analyzed. Cells per group from n = 3 mice. All data are the mean ± SEM.; Two-tailed Student’s t-test (a, c, d); ns, not significant.
