Extended Data Fig. 6: Supplementation of nucleotides and GSH sufficiently promotes GC B-cell formation and antigen-specific antibody production in response to NP-KLH or sheep blood red cells (SRBCs) antigen challenge in vivo.
From: Metabolic determinants of germinal center B cell formation and responses
a, Male Mthfd2+/+ and Mthfd2−/− mice (6 weeks old) (n = 6 per group) intraperitoneally injected with PBS (-) or purines and/or GSH every 2 days for 7 days were then immunized with NP-KLH. the serum was collected on day 21 and serum titers of total (NP25) of IgG2a and IgG2b, and high-affinity (NP7) of IgG2b were measured. b-e, Male Mthfd2+/+ and Mthfd2−/− mice (6 weeks old) (n = 6 per group) intraperitoneally injected (i.p.) with PBS, 100 mM purines plus 200 mM GSH every 2 days for 14 days were then immunized with 5×108 cells/mouse sheep blood red cells (SRBCs) by intraperitoneal injection (b). On day 14, spleens were collected and the percentage of GC B cells (Fas+GL7+, pregated on B220+ cells) were analyzed by FACS (c). The serum was collected on day21 and the relative titers of SRBCs-specific IgG1, IgM, IgG3, IgG2a and IgG2b were measured (d, e). All data are the mean ± SEM.; Two-tailed Student’s t-test (c-e); ns, not significant.
