Extended Data Fig. 1: GC B cells display increased one-carbon metabolism activity.
From: Metabolic determinants of germinal center B cell formation and responses
a, Schematic illustration of carbon transition from serine into purines through one-carbon metabolism catalyzed by SHMT2 and MTHFD2. Red circle represents 13C from serine. b, Mass isotopomer distribution (MID) of AMP, GMP, CMP and UMP in B cells cultured in medium containing 13C3-serine after 24 or 48 hours of activation with anti-CD40+IgM+IgG F(ab)2. Cells per group from n = 3 mice. c-f, Germinal center B cells, follicular B cells, marginal zone B cells and transitional B cells were isolated from splenocytes of 7-day NP-KLH-immunized C57BL/6 mice, and splenocytes or splenocytes pre-treated for 24 h with GSH or NAC were analyzed for glutathione levels by LC-MS (c), or ROS levels by DCF staining (d) and survival by PI staining (e) followed by FACS analysis. FACS plots are shown (f). n = 3 mice per group. All data are the mean ± SEM.; Two-tailed Student’s t-test (c-e); ns, not significant.
