Extended Data Fig. 2: Serine supports T cell-dependent GC B cell differentiation.
From: Metabolic determinants of germinal center B cell formation and responses
a,b, Levels of 13C-labled glycine (a), or methionine, CMP, TMP and UMP (b) in naive B cells activated by anti-CD40+IgG+IgM F(ab)2 in medium containing 400 µM 13C3-serine for the indicated time points. Cells per group from n = 3 mice c, d, Mouse naïve B cells activated by anti-CD40 + mIL4 for 72 hours in medium with (+) or without (-) serine and glycine (Serine/Glycine, SG) were stained with CFSE (c) or Ki67 antibody (d) and analyzed by FACS. Cells per group from n = 3 mice. e, f, Male C57BL/6 mice (6 weeks old) fed on serine/glycine-free diet were intravenously injected with PBS (-SG) or Serine/Glycine ( + SG) every 2 days, and immunized with or without (-) NP-KLH (e) or NP-LPS (f) on day 7 for another 7 days. The percentage of GC B cells and NP+ GC B cells were analyzed by FACS. n = 6 mice per group (-SG or +SG) or 3 mice for untreated (-) group. All data are the mean ± SEM.; Two-tailed Student’s t-test (d-f); ns, not significant.
