Extended Data Fig. 8: The alncRNA promotes degradation of EGFR in bladder cancer cell lines.
From: Engineering artificial non-coding RNAs for targeted protein degradation
(A-B) The protein levels of EGFR were analyzed in alncRNA-expressing BCa cells by WB. Data represent mean ± s.d. from three independent experiments. The P values were determined by a two-tailed unpaired Student’s t test. (C) The mRNA levels of EGFR were analyzed in alncRNA-expressing BCa cells by RT-qPCR. Data represent mean ± s.d. from three independent experiments. The P values were determined by a two-tailed unpaired Student’s t test. (D-E) The CCK-8 assay demonstrated the effect of alncRNA-degraded EGFR protein expression on the proliferation of bladder cancer cells (T24 and 5637). Data represent mean ± s.d. from three independent experiments. The P values were determined by a two-way ANOVA. (F-H) After alncRNA degraded EGFR protein expression, wound healing assays were utilized to assess the migratory abilities of two bladder cancer cells. Data represent mean ± s.d. from three independent experiments. The P values were determined by a two-tailed unpaired Student’s t test. (I) The caspase-3/ELISA assay demonstrates the impact of alncRNA targeting EGFR protein degradation on cell apoptosis (T24 and 5637). Data represent mean ± s.d. from three independent experiments. The P values were determined by a two-tailed unpaired Student’s t test. (J) The target genes of EGFR were downregulated by alncRNA. Data represent mean ± s.d. from three independent experiments. The P values were determined by a two-tailed unpaired Student’s t test.
