Fig. 1: N341 is a modified cytidine at position 34 of tRNAIle2 in plant organelles and bacteria.
From: A tRNA modification with aminovaleramide facilitates AUA decoding in protein synthesis
a, Secondary structures of tRNAIle2 from spinach chloroplasts (left) and mitochondria (right). The modifications other than N341 in chloroplast tRNAIle2 were reported previously29 and confirmed by LC–MS analyses (Extended Data Fig. 2a,b and Supplementary Table 1a,b). For mitochondrial tRNAIle2, the modifications other than Ψ were mapped by LC–MS analysis (Extended Data Fig. 2c and Supplementary Table 1c). b, LC–MS analysis of RNase A digests of spinach chloroplast tRNAIle2. The BPC (top) and XICs for L-containing (middle) and N341-containing (bottom) fragments are shown. c, CID spectrum of the N341-containing fragment of spinach chloroplast tRNAIle2 digested by RNase A. The product ions are indicated on the CID spectrum and assigned to the corresponding sequence. d, Nucleoside analyses of total RNA from various plants and bacterial species. The UV trace (top) and mass chromatograms detecting proton adducts of N341(middle) and L (bottom) are shown. The red arrows indicate the N341 peaks, and the blue arrows indicate the lysidine peaks. e, LC–MS analysis of RNase T1 digests of C. merolae chloroplast tRNAIle2. The BPC (top) and XICs for N341-containing (second), L-containing (third) and C-containing (bottom) fragments are shown. f. Nucleoside analysis of E. coli and V. cholerae tRNAIle2. MRM chromatograms detecting L (m/z 372 > 240) and N341 (m/z 342 > 210) are shown. g, LC–MS co-injection analyses of bacterial and plant N341. N341 from V. cholerae (top) and plants (middle), and a co-injected sample (bottom) were analyzed by HILIC (left) and ODS columns (right). XICs detecting N341 (m/z 342.2) are shown. BPC, base peak chromatogram; XICs, extracted ion chromatograms.
