Extended Data Fig. 5: Chi-REPAIRv2-mini and Chi-RESCUE-S-mini for efficient RNA base editing.
From: Compact RNA editors with natural miniature Cas13j nucleases
a. The schematic of three truncated ChiCas13j proteins. b. The mRNA cleavage efficacy of truncated ChiCas13j was analyzed by reporter assay. Statistical significance was assessed using two-tailed t-tests. p < 0.05 was considered to be statistically significant. c. The schematic of Chi-REPAIRv2-mini and Chi-RESCUE-S-mini RNA base editors by fusing truncated dChiCas13j with ADAR2dd variants. d. A-to-G editing efficiency of Chi-REPAIRv2-mini and REPAIRv2 on endogenous transcripts in HEK293T cells. e. A-to-G editing efficiency of Chi-RESCUE-S-mini and RESCUE-S on endogenous transcripts in HEK293T cells. f. C-to-U editing efficiency of Chi-RESCUE-S-mini and RESCUE-S on endogenous transcripts in HEK293T cells. All values shown are mean ± s.e.m. (n = 3 biological replicates, each average of 3 technical replicates).
