Extended Data Fig. 2: Collateral activity comparison of Cas13j proteins in HEK293T cells.
From: Compact RNA editors with natural miniature Cas13j nucleases
a. Schematics describing collateral activity detection system based on eGFP-transgenic HEK293T cells. b. Target knockdown and collateral activity for ChiCas13j targeting 16 mCherry reporters. c. Target knockdown and collateral activity for LepCas13j targeting 16 mCherry reporters. d. Target knockdown and collateral activity for ChiCas13j and LepCas13j targeting KRAS, RAF1, and NF2 endogenous mRNA. e. GFP MFI detection of ChiCas13j and LepCas13j targeting KRAS, RAF1, and NF2 endogenous mRNA. All values shown are mean ± s.e.m. (n = 3 biological replicates, each average of 3 technical replicates).
