Extended Data Fig. 8: Ole-AUTAB accelerates CD73 degradation.
From: Chemically engineered antibodies for autophagy-based receptor degradation
a, SDS-PAGE analysis of the Ole-AUTAB with Coomassie blue staining. b, Quantification of colocalization between mCherry-CD73 and LysoTracker in Fig. 4h (n = 15 cells per group, means ± s.d.). c, Live-cell flow cytometry analysis of surface CD73 levels in mCherry-CD73 stably expressing HeLa cells upon treatment with 200 nM L25K PEI, 100 nM Ole or 100 nM Ole-AUTAB for 12 h. Mean fluorescence intensity of surface CD73 relative to the untreated group was quantified (n = 3 biological replicates, means ± s.d.). d,e, Western blot analysis of CD73 in U87-MG cells upon treatment with increasing concentrations of Ole-AUTAB for 24 h (d), or upon treatment with 100 nM of Ole-AUTAB for different times (e). f, Western blotting to detect the CD73 degradation in Ole-AUTAB treated U87-MG cells in the presence or absence of bafilomycin A1 or chloroquine for 24 h. Statistical significance was calculated via unpaired two-tailed Student’s t-test (b, c).
