Extended Data Fig. 5: Influence of ProSeDMA variants on SAMURI activity.
From: Structure and catalytic activity of the SAM-utilizing ribozyme SAMURI
Gel images for kinetics graphs shown in Fig. 4d. Conditions: 10 pmol Cy5 labelled substrate RNA and 100 pmol ribozyme R4 in reaction buffer (50 mM HEPES, 120 mM KCl and 5 mM NaCl); after annealing (3 min at 95 °C, 10 min at 25 °C), 10 mM MgCl2 and 100 pmol respective cofactor were added (total reaction volume 10 μL). Incubation at 37 °C, 1 μL aliquots mixed with 4 μL of stop solution at each time point; kobs determined from pseudo-first order curve fit; Representative gel images of three independent replicates for all cofactors.
