Extended Data Fig. 4: Kinetics of SAMURI mutants.
From: Structure and catalytic activity of the SAM-utilizing ribozyme SAMURI
a. RNA oligonucleotides used for in-trans kinetic assays in a schematic depiction according to the structure, with mutated nucleotides and base-pairs indicated. Sequence information for RNA substrate oligonucleotides R1, R5, R6, and ribozymes R7-R15 are provided in Supplementary Table 1. The box on the lower left details the suggested H-bonding interactions of the mutant ribozyme tested for alkylation of guanosine or inosine instead of adenosine. b. Summary of rate constants determined at two different ProSeDMA concentrations of 10 µM and 50 µM for the slower mutants. At least two independent replicates were performed. Exemplary gel images are shown in (c).
