Extended Data Fig. 5: ARAF’s N terminus mediates ARAF-RAS granule formation.
From: Plasma membrane-associated ARAF condensates fuel RAS-related cancer drug resistance
a, 5 μM mCherry-RAS proteins were incubated with 5 μM ARAF 255 ∆N-GFP for 60 minutes. Images were taken. Scale bar, 5 μm. b, 5 μM mCherry-RAS proteins were incubated with 5 μM ARAF 255 or ARAF 255 ∆N for 60 minutes. Images were taken (left), scale bar, 5 μm. Granule numbers were quantified, n = 3 (right). Error bars, mean ± SD (biological replicates), unpaired two-tailed Student’s t-test. c, 5 μM mCherry-RAS proteins were incubated with 5 μM ARAF 255BF-GFP for 60 minutes. BF, RAS binding deficient. Images were taken. Scale bar, 5 μm. d, 5 μM mCherry-RAS proteins were incubated with 5 μM GFP-NF1 GRD for 60 minutes. Scale bar, 5 μm. e, f, 2.5 μM Alexa fluor 647-labeled ARAF 255 were incubated with 2.5 μM indicated RAS proteins (e) or GFP-NF1 GRD (f) for 60 minutes. Scale bar, 5 μm.
