Extended Data Fig. 6: Plasma membrane assembly of ARAF drives RAS activation.
From: Plasma membrane-associated ARAF condensates fuel RAS-related cancer drug resistance
a, SKBR3 cells co-transfected with indicated plasmids were subjected for imaging. Scale bar, 5 μm. b, NIH3T3 cells transfected with the indicated plasmids were subjected for active RAS pulldown (upper panel). The corresponding N-terminal disorder probabilities of ARAF mutants were shown in the lower panel. c, NIH3T3 cells stably expressing RAS variants were transfected with ARAF. RAS-GTP levels were examined (left) and quantified, n = 3 (right). Immunoblots are representative of three independent experiments in b, c. Error bars denote the mean ± SD (biological replicates), and statistical analyses were performed using unpaired two-tailed Student’s t-test in c.
