Extended Data Fig. 7: Plasma membrane assembly of ARAF drives RAS activation.
From: Plasma membrane-associated ARAF condensates fuel RAS-related cancer drug resistance
a, b, Immunofluorescent staining in SKBR3 cells transfected with indicated constructs. Images were taken (a), percentages of cells with PM RAS signals were quantified, n = 3 (b). None, no membrane RAS signal; partial PM, discontinuous signals covering <50% of PM area; uniform PM, continuous signals covering > 60% of PM area. Scale bar, 5 μm. c, Immunofluorescent staining in SKBR3 cells transfected with indicated constructs. Images were taken (left), percentages of cells with PM RAS signals were quantified, n = 3 (right). Scale bar, 5 μm. d, SKBR3 cells transfected with the indicated plasmids were subjected for imaging (left), scale bar, 5 μm. Percentages of cells with PM signals were quantified, n = 3 (right). Error bars, mean ± SD (biological replicates), unpaired two-tailed Student’s t-test. e, 293FT cells transfected with the indicated plasmids were subjected for active RAS pulldown. Immunoblots are representative of three independent experiments.
