Fig. 4: Effect of CCM treatment on macrophage gene expression signature.
From: Pharmacological targeting of BMAL1 modulates circadian and immune pathways
a, Volcano plots display differentially expressed genes resulting from CCM treatment in WT BMDMs in both basal and stimulated states (from treatment with LPS). Female mice were used for all conditions (n = 3, WT; n = 6, KO). Differential expression analysis used DESeq2, and P values were adjusted for multiple comparisons using the Benjamini–Hochberg procedure to control for FDRs. b, Gene Ontology (GO) for the 1,391 genes modulated by CCM in WT-stimulated macrophages. The inserted Venn diagram shows the numbers of genes regulated in the WT-stimulated macrophages, intersecting with the genes regulated in the BMAL1-KO-stimulated macrophages. c, Genes involved in phagocytosis are predominantly downregulated by CCM in macrophages (n = 3, biological replicates). Data are presented as mean values ± s.d. FC, fold change.
