Extended Data Fig. 5: Disruption of the Henry regulatory circuit shifts temporal oscillations in phospholipids.
From: Temporal oscillation of phospholipids promotes metabolic efficiency
a, Representative Opi1–GFP fluorescence images at different YMC phases. Data shown are representative of two independent experiments with similar results. b, Relative mRNA levels of phospholipid biosynthesis genes in WT and opi1Δ cells during the YMC. Data are presented as mean ± s.d. (n = 27 biologically independent samples). c, PE, PC, PI and PS abundance (% PL) in WT (n = 22 biologically independent samples) and opi1Δ cells (n = 26 biologically independent samples) across the YMC. Data are presented as mean ± s.d. d, Hierarchical clustering of phospholipids in opi1Δ cells across the YMC. Data normalized to total ion counts, log-transformed and clustered (Spearman rank). Sankey diagram shows phospholipid class distribution. e, Top, sine waves representing major phospholipid classes in the opi1Δ mutant. Solid lines indicate fitting of statistical significance (P < 0.05), while dashed lines represent nonsignificant fitting. Bottom, summary table of fitting parameters. Shaded areas are used to highlight a budding cycle. f,g, Comparison of PI (f) and PS (g) oscillations between WT and the opi1Δ mutant, showing differences in Ttrough and amplitude across their molecular species. Statistical analyses were performed using an unpaired t test in b and c. Statistical analyses were performed using a two-sided t test in e. Shaded areas are used to highlight a budding cycle.
