Extended Data Fig. 3: RIL to quantify the turnover and synthesis of phospholipids.
From: Temporal oscillation of phospholipids promotes metabolic efficiency
a, Schematic of the TAG biosynthesis pathway with genes at respective steps highlighted in red. b, Heatmap depicting the oscillatory expression of genes involved in TAG biosynthesis across the YMC. The heatmap is generated using RNA-seq data previously published31. c, Relative abundance of indicated phospholipid classes with acyl chains, polar head groups and the glycerol backbone individually or combinatorially labeled with 13C and 12C after two rounds of doubling in the RIL experiment. Relative abundance was calculated by normalizing to the total amount of corresponding lipid classes with full 13C labeling before the RIL switch. d, Sine waves fitting of PE, PC, PI and PS abundance in the dga1Δlro1Δ mutant of the YMC, characterized by their acyl composition. Solid lines indicate fitting of statistical significance (P < 0.05), while dashed lines represent nonsignificant fitting. Statistical analyses were performed using a two-sided t-test in d. Shaded areas are used to highlight a budding cycle.
