Extended Data Fig. 3: Recruitment of HA-TDP-43-FKBP to PML NBs.
From: Induced proximity to PML protects TDP-43 from aggregation via SUMO–ubiquitin networks
a, Statistical analysis of the immunofluorescence images shown in Fig. 3c. Enrichment of HA–TDP-43–FKBP in Myc-positive PML nuclear bodies (leftmost plot) and the correlation of the indicated signal pairs within the nucleus were analyzed. P values of two-tailed, unpaired Student’s t-tests are shown. At least 55 cells were measured per condition. b, HeLa cells were transfected with WT FRB–PML–Myc (WT V57,60S or L73E) and HA–TDP-43–FKBP for 24 h and either treated DMSO or rapamycin (100 nM, 4 h). Insets are marked with yellow squares. Scale bar, 2 µm. c, HeLa cells were transfected with WT FRB–PML–Myc and HA–TDP-43–FKBP for 24 h and either treated with DMSO or rapamycin (100 nM, 4 h). FRB–PML–Myc and PIAS1 were detected with anti-Myc and anti-PIAS1 antibodies and are shown in red and green, respectively. Inset areas are indicated with yellow squares. Scale bar, 10 µm.
