Extended Data Fig. 4: The levels of PHGDH and PSAT are unchanged in TLE.
From: Pharmacological inhibition of PSPH reduces serine levels and epileptic seizures
(a-d) Screening of siRNAs against PHGDH, PSAT1 and PSPH. Immunoblot (a) and statistical analysis (b-d) of cultures of astrocytes transfected with the indicated siRNA. (e, f) Statistical analysis of L-serine levels in the cell supernatant (e) and lysates (f) of cultures of astrocytes following PSPH, PSAT1 or PHGDH KD with another set of siRNAs (n = 4). (g, h) Immunofluorescence staining for PHGDH (g) or PSAT1 (h) in hippocampus samples surgically resected from TLE patients with hippocampal sclerosis (TLE-HS) and autopsy controls without neurological disorders (control). (i, j) Immunofluorescence staining of PHGDH (i) or PSAT1 (j) in hippocampus samples from control and TLE mice (4 weeks post-KA-induced SE); scale bar = 50 μm. Statistical analyses were performed with one-way ANOVA followed by Tukey’s post hoc comparison test (e, f) or two-tailed Student’s t test (g-j).
