Extended Data Fig. 8: Cryo-EM analysis for ex vivo fibrils extracted from AD1 by using water-based extraction method.
From: Unraveling Alzheimer’s complexity with a distinct Aβ42 fibril type and specific AV-45 binding
a Workflow of the water-based fibril extraction procedure. Solid arrows depict the steps used in the next step. P5 was used for cryo-EM analysis. b NS-TEM images of the supernatants of the 12-time repeat wash highlighted in (a). Samples 9–12 framed with a red frame were combined as S4. c Representative cryo-EM micrograph of P5 from one of 16,305 movies. d The 3 most populated 2D class averages of fibrils in P5. Fibril polymorph was determined based on the crossover distance (pitch), fibril width and morphology. Type I fibrils are labeled in green; Type III fibrils are labeled in orange. Constructed using 2× binned particles of the full set of segments after the removal of picking artifacts. The box size is ∼87 nm.
