Fig. 2: Lack of PtdIns(4,5)P2-containing liposome binding in NC-site-mutant βarr.
From: Membrane-domain compartmentalization of active GPCRs by β-arrestins through PtdIns(4,5)P2 binding
a, Schematic representation of the liposome-binding assay. b, Assessment of βarr and PtdIns(4,5)P2 interactions through the precipitation of liposomes containing PtdIns(4,5)P2 and βarr. SDS–PAGE was performed to analyze the results of βarr1 mixed with liposomes under various conditions: no liposome (−), 100% PC and a mixture of 10% PtdIns(4,5)P2 + 90% PC. c, Quantification of the liposome-binding assay. The values of percentage bound (pellet/total) are shown in a bar graph. Bars and error bars represent the mean and s.e.m., respectively, of three independent experiments. For the statistical analyses, data were analyzed by one-way ANOVA followed by the Dunnett’s test for multiple-comparison analysis. ***P < 0.001 versus WT. The schematic was created with BioRender.com.
